DE 22

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

M.Sc. DEGREE EXAMINATION – ZOOLOGY

FIRST SEMESTER – APRIL 2008

ZO 1811 – HISTOCHEMISTRY & MICROTECHNIQUE

 

 

 

Date : 06-05-08                  Dept. No.                                        Max. : 100 Marks

Time : 1:00 – 4:00

PART – A

Answer ALL the questions. Each question carries TWO marks.                       (10 x 2 = 20 Marks)

1. Comment on vital staining.
2. What is photomicrography?
3. What is collagen?
4. What are Mordants and Lakes?
5. What is Metachromasia?
6. Comment on autopsy.
7. Comment on stoke shift.
8. Write a note on Mayer’s albumen.
9. Differentiate preservative from fixative.
10. Expand: ICMR& STEM.

PART – B

Answer any FOUR questions. Each question carries TEN marks.       (4 x 10 = 40 Marks)

11. Give an account on Fluorescent microscope and its applications.
12. Describe the procedure involved in dry mount of insects.
13. Classify lipids and describe Oil Red O method.
14. Give an account on Cryostat and its applications.
15. Describe the procedure involved in mounting of soil microarthropods.
16. Give an account on the identification of DNA by Feulgen reaction.

 

PART – C

Answer any TWO questions. Each question carries TWENTY marks.           (2 x 20 = 40 Marks)

17. What is Ultramicrotome? Describe the steps involved in ultramicrotomy.
18. Give an account on Autoradiography and its applications.
19. Explain the working principle of SEM and its sample preparation.
20. Write notes on:
    1. Classification of proteins and Ninhydrin-Schiff method.
    2. Explain the different steps of Haemotoxylin – Eosin staining procedure involved in Paraffin microtomy.

 

Go To Main page

Latest Govt Job & Exam Updates:

View Full List ...